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L-Malic Acid UniFlex Reagent (=LMA-F): An enzymatic test kit for determination of L-malic acid in foods, beverages and other samples.
Contains read-to-use Buffer, NAD Subtstrate solution, two enzyme suspensions (Malate Dehydrogenase and GOT), as well as a L-Malic Acid Standard.
L-malic acid is present in grapes, apples and other fruit and vegetables. In grape juice, its concentration drops from (as high as) 8 to perhaps 1 g/L in grape must as the ripening process proceeds; L-Malic levels, along with sugar concentration, are typically used to gauge ripeness for wine production. Up to 30% of the malic acid may be consumed by yeast fermentation. A secondary fermentation is typical in wine; L-malic acid is converted to L-lactic acid and carbon dioxide by lactic bacteria. Mali-lactic fermentation can be prevented by filtration and increased sulfite.
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